王旭东

浙江工业大学药学院副教授，硕士生导师，生物药与生物制药研究所师生联合第一党支部书记，曾任生物制药系主任。

大连理工大学本科、博士 (硕博连读) 和博士后。

1. 教学工作

主讲生物制药专业本科生课程《生物制药专业导论》、绿色制药专业本科生课程《绿色制药分离工程》、全校本科生通识 (创新创业) 课程《创造性思维与创新方法》。

2.科研工作 

研究方向:(1) 蛋白多肽及其偶联药物研发；(2) 微生物发酵及其产物分离；（3）体外多酶催化与生物合成。

主持国家自然科学基金青年科学基金项目1 项、浙江省自然科学基金项目1 项、浙江省“尖兵”科技计划项目子课题1 项和重大横向项目1 项，参与国家科技支撑计划、国家自然科学基金面上项目、浙江省重点研发项目和企业横向课题10 余项。在Journal of Medicinal Chemistry、Separation and Purification Technology、Industrial & Engineering Chemistry Research、Biochemical Engineering Journal 和Process Biochemistry 等期刊上累计发表学术论文50余篇，申请国家发明专利20余件 (已授权10余件)。

期刊论文 (# 共同一作；*通讯作者）

51. Huitian Lin^#, Cheng Zhu^#, Tianfeng Shang^#, Ning Zhu, Kang Lin, Chengyun Zhang, Xiang Shao*, Xu-Dong Wang*, Hongliang Duan*. HighPlay：Cyclic Peptide Sequence Design Based on Reinforcement Learning and Protein Structure Prediction，Journal of Medicinal Chemistry, 2025, 68(11): 12047–12057. 

50. Yunhui Zhang, Bowei Zhang, Xiaoxia Liu, Xiang Li, Fan Wei, Lingda Zhao, Huan Lv, Xudong Wang, Jin Wang, Jing Wu, Yan Zhang, Shuo Wang*. Gastrointestinal distribution and in vitro-microbial biotransformation of chlorogenic acid, Food Science and Human Wellness, 2024, https://doi.org/10.26599/FSHW.2024.9250312

49. Zhihao Su, Yejian Wu, Kaiqiang Cao, Jie Du, Lujing Cao, Zhipeng Wu, Xinyi Wu, Xinqiao Wang,Ying Song, Xudong Wang*, Hongliang Duan*. APEX-pHLA: A novel method for accurate predictionof the binding between exogenous short peptides and HLA class I molecules, Methods, 2024, 228: 38-47.

48. Tianqi Cai, Xiaoxue Xu, Ling Dong, Shufei Liang, Meiling Xin, Tianqi Wang, Tianxing Li, Xudong Wang, Weilong Zheng, Chao Wang, Zhengbao Xu, Meng Wang, Xinhua Song, Lingru Li*, Jingda Li*, Wenlong Sun*.Oroxin A from Oroxylum indicum improves disordered lipid metabolism by inhibiting SREBPs in oleic acid-induced HepG2 cells and high-fat diet-fed non-insulin-resistant rats, Heliyon, 2024, 10 (7): e29168.

47. Xu-Dong Wang*, Zhi-Hao Su, Jie Du, Wei-Jia Yu, Wen-Long Sun*. Site-selective Fatty Acid Chain Conjugation of the N-terminus of the Recombinant Human Granulocyte Colony-Stimulating Factor，Frontiers in Bioengineering and Biotechnology，2024, 12:1360506.

46. Shu-Chang Wang, Xu-Dong Wang, Xin-Nan Teng, Jian-Ying Dai, Yue-Sheng Dong, Heng-Li Yuan, Zhi-Long Xiu*. Fatty acid chain modification of loxenatide and its kinetics in a continuous flow microchannel reactor, Process Biochemistry, 2023, 124: 259-268.

45. Xu-Dong Wang*, Wei-Jia Yu, Jia-Hui Liu, Jie Du, Kang-Nan Chen, Qin-Qin Hu, Wen-Long Sun*, Guo-Qing Ying*. Preparation and characterization of site-specific fatty chain-modified recombinant human granulocyte colony stimulating factor, Frontiers in Bioengineering and Biotechnology, 2022, 10: 923059.

44. 王旭东，刘家辉，陈康楠，梅建凤，易喻，应国清*. 长效化重组人粒细胞集落刺激因子的研究进展，药学学报，2022, 57(04): 875-883.

43. Jingda Li*, Tao Xiong, Tianqi Wang, Meng Wang, Chao Wang, Fuyuan Yang , Xudong Wang, Zhijian Tan*, Wenlong Sun*.Baicalein targets CD36 to prevent foam cell formation by suppressing the excessive uptake of oxLDL and accelerating ABCA1-mediated cholesterol efflux in oxLDL-induced THP-1 macrophages,Journal of Functional Foods, 2022,97: 105253.

42. Shu-Chang Wang, Xin-Nan Teng, Xu-Dong Wang, Yue-Sheng Dong, Heng-Li Yuan, Zhi-Long Xiu*. Recycling reaction and separation for FACylation of loxenatide by trade-off between miscibility and immiscibility of reactants and product in methanol solution, Journal of Chromatography A, 2022, 1676:463239.

41. Jianfeng Mei, Shaofen Cai, Yu Yi, Yanlu Zhang, Xudong Wang, Guoqing Ying*. Study of the fibrinolytic activity of serrapeptase and its in vitro thrombolytic effects, Brazilian Journal of Pharmaceutical Sciences, 2022, 58: e201004.

40. Yu Yi*, Ting Huang, Yunxia Xu, Jianfeng Mei, Yanlu Zhang, Xudong Wang, Jianshu Chen and Guoqing Ying*. Preparation and properties of N-glycosylated chitosan_polyvinyl alcohol hydrogels for use in wound dressings, Journal of Applied Biomaterials & Functional Materials, 2022, DOI: 10.1177/22808000221101809.

38. 潘多涛，王旭东，史洪岩，修志龙*．生物基 1， 3-丙二醇连续发酵过程的稳态分析与反馈控制，化工学报，2022，73(5): 2094-2100.

37. Duo-Tao Pan, Xu-Dong Wang, Jia-Bing Wang, Hong-Yan Shi, Guo-Gang Wang, Zhi-Long Xiu*. Optimization and feedback control system of dilution rate for 1,3-propanediol in two-stage fermentation, Biotechnology Progress, 2022,38:e3225.

36. Huiju Zhang, Min Shi, Shuting Su,  Sikai Zheng,  Miao Wang,  Jun Lv,  Xudong Wang,  Quan Yang, Hongyang Gao*. Whole-genome methylation analysis reveals epigenetic differences in the occurrence and recovery of hyperhydricity in Dendrobium officinale plantlets, In Vitro Cellular & Developmental Biology – Plant, 2022, 58:290-301.

35. Yu Yi*, Jianming Hu, Shenwei Ding, Jianfeng Mei, Xudong Wang, Yanlu Zhang, Jianshu Chen, Guoqing Ying*. A preparation strategy for protein-oriented immobilized silica magnetic beads with spy chemistry for ligand fishing,Journal of Pharmaceutical Analysis, 2022, 12:415-423.

34. Jianfeng Mei, Xia Wu, Shujing Zheng,Yu Yi, Xudong Wang, Guoqing Ying*. Production of bilirubin by biotransformation of biliverdin using recombinant Escherichia coli cells, Bioprocess and Biosystems Engineering, 2022, 45(3):563-571.

33. Xu-Dong Wang^#, Chun-Yan Hu ^#, Chao Qin, Yue-Sheng Dong, Guo-Qing Ying, Zhi-Long Xiu*, Zhi-Guo Su. Simultaneous solid–liquid separation and primary purification of clavulanic acid from fermentation broth of Streptomyces clavuligerus using salting-out extraction system, Engineering in Life Sciences, 2021, 21:643–652.

32. Wen-Long Sun, Xin-Yu Li, Hao-Yue Dou, Xu-Dong Wang, Jing-Da Li, Liang Shen*, Hong-Fang Ji*. Myricetin supplementation decreases hepatic lipid synthesis and inflammation by modulating gut microbiota, Cell Reports, 2021, 36(9):109641.

31. Jingda Li*, Tianqi Wang, Panpan Liu, Fuyuan Yang, Xudong Wang, Weilong Zheng, Wenlong Sun*. Hesperetin ameliorates hepatic oxidative stress and inflammation via the PI3K/AKT-Nrf2-ARE pathway in oleic acid-induced HepG2 cells and a rat model of high-fat diet-induced NAFLD,Food & Function, 2021,12, 3898-3918.

30. Wenlong Sun*, Panpan Liu, Bendong Yang, Meng Wang, Tianqi Wang, Wenbo Sun, Xudong Wang, Weilong Zheng, Xinhua Song*, Jingda Li*. A network pharmacology approach: inhibition of the NF-κB signaling pathway contributes to the NASH preventative effect of an Oroxylum indicum seed extract in oleic acid-stimulated HepG2 cells and high-fat diet-fed rats, Phytomedicine, 2021, 88, 153498.

29. Jianfeng Mei, Xiaoyun Zhao, Yu Yi, Yanlu Zhang, Xudong Wang, Guoqing Ying*. Preparation of astaxanthin by lipase-catalyzed hydrolysis from its esters in a slug-flow microchannel reactor, Process Biochemistry, 2020, 98: 241-246.

28. Wenlong Sun*, Panpan Liu, Tianqi Wang, Xu-Dong Wang, Weilong Zheng, Jingda Li*. Baicalein reduced hepatic fat accumulation by activating AMPK in oleic acid-induced HepG2 cells and high-fat diet-induced non-insulin resistant mice,Food & Function, 2020, 11(1): 711-721.

27. Shu-Chang Wang ^#, Xu-Dong Wang^#, Xin-Nan Teng, Zhi-Long Xiu^*. Chemical modification of recombinant hirudin with palmitic acid in mixed aqueous-organic solutions,Process Biochemistry, 2019, 55:85-92.

26. Liaqat Zeb, Xu-Dong Wang, Wei-Long Zheng, Xin-Nan Teng, Muhammad Shafiq, Ying Mu, Zhan-You Chi, Zhi-Long Xiu*. Microwave-assisted three-liquid-phase salting-out extraction of docosahexaenoic acid (DHA)-rich oil from fermentation broths of Schizochytrium limacinium SR21, Food and Bioproducts Processing, 2019, 118: 237-247.

25. Yue-Sheng Dong*, Fan Yang, Wen-Cai Liu, Xu-Dong Wang, Yan Xing, Zhi-Long Xiu. Separation and purification of wortmannilactone analogues by three-liquid-phase salting-out extraction coupled with column chromatography, Separation and Purification Technology, 2019,224:397-404.

24. Duo-Tao Pan, Xu-Dong Wang, Hong-Yan Shi, De-Cheng Yuan, Zhi-Long Xiu^*. Ensemble optimization of microbial conversion of glycerol into 1, 3-propanediol by Klebsiella pneumoniae, Journal of Biotechnology, 2019, 301: 68-78.

23. Guo-Qing Ying, Xing-Ru Lu, Jian-Feng Mei, Yan-Lu Zhang, Jian-Shu Chen, Xu-Dong Wang, Zhi-Ming Ou, Yu Yi*. A structure-activity relationship of a thrombin-binding aptamer containing LNA in novel sites, Bioorganic & Medicinal Chemistry, 2019, 27(14): 3201-3207.

22. Xu-Dong Wang, Ning-Ning Wei, Shu-Chang Wang, Heng-Li Yuan, Feng-Ying Zhang, Zhi-Long Xiu*. Kinetic optimization and scale up of site-specific thiol-PEGylation of loxenatide from laboratory to pilot scale, Industrial & Engineering Chemistry Research, 2018, 57 (44): 14915-14925.

21. Xudong Wang, Shuchang Wang, Duotao Pan, Kairong Qin, Hengli Yuan, Fengying Zhang, Yundong Sun, Zhilong Xiu^*. Combined optimization of N-terminal site-specific PEGylation of recombinant hirudin using response surface methodology and kinetic analysis, Engineering in Life Sciences, 2018, 18 (8): 611-621.

20. 胡春燕，王旭东^*，秦超，董悦生，修志龙. 克拉维酸钾一步结晶工艺优化，高校化学工程学报，2018, 32 (4) : 910-917.

19. Ling Yan, Ya-Qin Sun, Xu-Dong Wang, Hong-Xin Fu, Ying Mu, Zhi-Long Xiu^*. Partition behavior of monocarboxylic acids in salting-out extraction systems of monohydric alcohols and dipotassium phosphate, Separation and Purification Technology, 2018, 199:351-358.

18. Weilong Zheng, Jingda Li, Xudong Wang, Yanping Yuan, Jianing Zhang, Zhilong Xiu^*. Effects of Antarctic krill docosahexaenoic acid on MCF-7 cell migration and invasion induced by the interaction of CD95 with caveolin-1, Life Sciences, 2018, 192: 270-277.

17. Duo-Tao Pan，Xu-Dong Wang，Hong-Yan Shi，De-Cheng Yuan, Zhi-Long Xiu^*. Dynamic flux balance analysis for microbial conversion of glycerol into 1,3-propanediol by Klebsiella pneumoniae, Bioprocess and Biosystems Engineering, 2018, 41(12):1793-1805.

16. 鄢凌, 傅宏鑫, 王旭东, 孙亚琴, 戴建英, 修志龙^*. 生物基有机酸的提取分离技术研究进展,过程工程学报, 2018, 18(1): 1-10.

15. Xudong Wang, Li Lv, Kairong Qin, Hengli Yuan, Fengying Zhang, Guohui Chen, Zhilong Xiu^*. Effects of linear and branched polyethylene glycol on PEGylation of recombinant hirudin: Reaction kinetics and in vitro and in vivo bioactivities, Process Biochemistry, 2017, 63:154-162.

14. Hongxin Fu^#, Xudong Wang^#, Yaqin Sun, Ling Yan, Juntao Shen, Jufang Wang, Shang-Tian Yang, Zhilong Xiu^*. Effects of salting-out and salting-out extraction on the separation of butyric acid, Separation and Purification Technology, 2017, 180:44-50.

13. Xudong Wang, Xueqin Li, Jun Zhao, Li Lv, Kairong Qin, Hengli Yuan, Zhi-Long Xiu^*. In situ PEGylation of recombinant hirudin on an anion exchange chromatography column, Process Biochemistry, 2017, 55:162-171.

12. Weilong Zheng, Xudong Wang, Wenjing Cao, Bowen Yang, Ying Mu, Yuesheng Dong, Zhilong Xiu^*. E-configuration structures of EPA and DHA derived from Euphausia superba and their significant inhibitive effects on growth of human cancer cell lines in vitro, Prostaglandins, Leukotrienes & Essential Fatty Acids, 2017, 117: 47-53.

11. Juntao Shen, Li Lv, Xudong Wang, Zhilong Xiu^*, Guoqiang Chen. Comparative analysis of CRISPR-Cas systems in Klebsiella genomes. Journal of Basic Microbiology, 2017, 57: 325-336.

10. 胡春燕，秦超，王旭东，董悦生，修志龙^*,李树有, 冯涛, 王启斌. 克拉维酸在乙酸乙酯中的稳定性, 高校化学工程学报, 2017, 31(2):270-275.

09. 姜莉莉，周瑾洁，王旭东，孙亚琴，修志龙^*. 微生物菌群发酵生产化学品的研究进展，生物工程学报, 2016, 32(11): 1496-1506.

08. 周瑾洁，王旭东，孙亚琴，修志龙^*. 生物基化学品的微生物电合成研究, 化工进展, 2016, 35(10):3005-3015.

07. Xu-Dong Wang, Hu Teng, Jing-Jing Hu, Zhi-Long Xiu^*. PEGylation of recombinant hirudin in mixed aqueous-organic solutions, Process Biochemistry, 2015, 50: 367-377.

06. Xudong Wang, Jingjing Hu, Duotao Pan, Hu Teng, Zhilong Xiu^*. PEGylation kinetics of recombinant hirudin and its application for the production of PEGylated HV2 species, Biochemical Engineering Journal, 2014, 85: 38-48.

05. 王旭东，李晓晖，苏志国，修志龙^*. 蛋白质药物的聚乙二醇定点修饰策略与最佳位点，中国生物工程杂志，2010, 30 (4) : 101-109.

04. 孙丽慧，戴建英，王旭东，修志龙^*. 两株Klebsiella pneumoniae 菌发酵生产2,3-丁二醇的比较，过程工程学报, 2010, 10 (3):588-592.

03. 孙丽慧，戴建英，王旭东，修志龙^*. 发酵条件对产2,3-丁二醇Klebsiella pneumoniae 代谢的影响，食品与发酵工业, 2010, 36 (8):6-11.

02. Li-Hui Sun, Xu-Dong Wang, Jian-Ying Dai, Zhi-Long Xiu^*. Microbial production of 2,3-butanediol from Jerusalem artichoke tubers by Klebsiella pneumoniae, Applied Microbiology and Biotechnology, 2009, 82: 847-852.

01. 孙丽慧，王旭东，戴建英，修志龙^*. Klebsiella pneumoniae 发酵菊芋块茎生产2,3-丁二醇的初步研究，过程工程学报, 2009, 9 (1):161-164.

01. 浙江省“尖兵”科技计划项目(2025C01094)：大宗化学品低碳生物制造技术研发，2025/01-2026/12，承担课题任务，参与单位负责人（项目牵头单位西湖大学）。

02. 国家自然科学基金青年基金项目(21606038)：超大孔离子交换介质辅助聚乙二醇修饰水蛭素的动力学研究，2017/01-2019/12，负责人.

03. 浙江省自然科学基金探索项目(Y类) (LY21B060007)：脂肪酸反胶团原位修饰蛋白药物的新反应模式及其过程强化机制研究，2021/01-2023/12，负责人.

04. 企业重大横向项目，2021/10-2023/10，负责人.

05. 企业横向项目，2024/05-2027/04，负责人.

06. 企业横向项目，2021/09-2021/11，负责人.

07. 浙江省重点研发计划(No. 2021C03088)：新型生物药物开发研究-抗体融合蛋白分子筛选评价技术平台建立及新型抗体融合蛋白GMA106临床前研究，2021/01 -2023/12，参与.

08. 国家科技支撑计划项目(2015BAD16B0904)：北方特色浆果绿色防腐保鲜技术研发集成与应用，2015/04-2018/03，参与.

09. 国家自然科学基金面上项目(81172966)：蠕虫复合体I沃特曼内酯类化合物及其作用机理研究，2012/01-2015/12，参与.

10. 国家自然科学基金面上项目(81072590)：离子交换柱原位PEG修饰蛋白药物的微环境工程研究，2011/01-2013/12，参与.

11. 大连理工大学基本科研业务费专项项目(交叉探索科研专题, DUT18JC36)，2018/01-2019/12，参与.

12. 企业横向项目，2016/07-2017/07，参与.

13. 企业横向项目，2016.07-2016/12，参与.

14. 企业横向项目，2015/11-2016/12，参与.