蔡雪

副教授，博导，浙江工业大学生物工程学院教师。教育部全国高校黄大年式教师团队成员，浙江省生物有机合成技术重点实验室成员，浙江工业大学郑裕国院士创新团队成员。先后主持了国家自然科学基金青年基金项目、浙江省自然科学基金等国家级、省部级项目、企业横向等5项。在ACS Sus Chem Eng、J Agri Food Chem、Bioresour Technol等知名期刊发表SCI收录论文38余篇，申请中国发明专利30余项，授权中国发明专利10件，申请国际PCT专利2件。

本科生：生物工艺学原理、合成生物学技术前沿与应用、现代生物技术进展

研究生：生物催化工艺学、合成生物学

1. 浙江省第九届“互联网+”大学生创新创业大赛银奖（指导教师排名1/10），2023年

2. 第九届杭州市大学生科技创新大赛优秀创新奖（指导教师排名1/1），2023年

3. 浙江工业大学第十一届大学生节能减排社会实践与科技竞赛一等奖1项（指导教师排名1/2），2023年

4. 第十五届“运河杯”浙江工业大学大学生创业大赛暨第九届“互联网+”大学生创新创业大赛选拔赛铜奖1项（指导教师排名1/6）和金奖1项（指导教师排名2/6）、铜奖1项（指导教师排名4/6），2023年

5. 指导本科生徐雨欢获国家创业训练项目立项1项（1/5），2023年

6. 浙江工业大学“运河杯”大学生课外学术科技基金立项项目1项（指导硕士生林振明排名1/3），2023年

• 主要研究项目

代表性论文论著

[1] Xue Cai, Jiang Hua, Zhen-Ming Lin, Chen-Yang Sun, Chang-Hui Hu, Xiaojian Zhang, Ji-Dong Shen, Hai-Yan Zhou, Hong-Yan Wang, Kai-Qian Chen, De-Shui Chen, Xin-Ping Cheng, Mian Li, Zhi-Qiang Liu*, Yu-Guo Zheng. Enhanced enzymatic hydrolysis of high-solid content corncobs by a lytic polysaccharide monooxygenase from Podospora anserina S mat+ for valuable monosaccharides. ACS Sustainable Chemistry & Engineering, 2023, 11: 9858–9867. （唯一第一作者）

[2] Xue Cai, Xue Shi, Si-Qi Liu, Yu Qiang, Ji-Dong Shen, Bo Zhang, Zhi-Qiang Liu*, Yu-Guo Zheng. Hot spot-based engineering of ketopantoate hydroxymethyltransferase for the improvement of d-pantothenic acid production in Escherichia coli. Journal of Biotechnology, 2023, 364: 40–49.（唯一第一作者）

[3] Xue Cai, Jiang-Wei Shen, Yu Qiang, Jing Hua, Zhang-Qi Ma, Zhi-Qiang Liu*, Yu-Guo Zheng. Efficient activity enhancement of a lipase from Sporisorium reilianum for the synthesis of a moxifloxacin chiral intermediate via rational design. Engineering, 2022, 19: 207–216.（唯一第一作者）

[4] Xue Cai, Chang-Hui Hu, Jing Wang, Xu-Hao Zeng, Jia-Xing Luo, Mian Li, Zhi-Qiang Liu*, Yu-Guo Zheng. Efficient high-solids enzymatic hydrolysis of corncobs by an acidic pretreatment and a fed-batch feeding mode. Bioresource Technology, 2021, 326: 124768. （唯一第一作者）

[5] Xue Cai, Jie-Yi Jin, Bo Zhang, Zhi-Qiang Liu*, Yu-Guo Zheng. Improvement of cordycepin production by an isolated Paecilomyces hepiali mutant from combinatorial mutation breeding and medium screening. Bioprocess and Biosystems Engineering, 2021, 44 (11): 2387–2398. （唯一第一作者）

[6] Qi-Le Zuo^#, Xue Cai^#, Xiao-Yang Zheng, De-Shui Chen, Mian Li, Zhi-Qiang Liu, Kai-Qian Chen, Fei-Fei Han, Xuan Zhu*. Influences of xylitol consumption at different dosages on intestinal tissues and gut microbiota in rats. Journal of Agricultural and Food Chemistry, 2021, 69(40): 12002–12011.（共同第一作者）

[7] Xue Cai, Ines Seitl, Wanmeng Mu, Tao Zhang, Timo Stressler, Lutz Fischer*, Bo Jiang*. Characterization of a recombinant trehalose synthase from Arthrobacter chlorophenolicus and its unique kinetics indicating a substrate cooperativity. Applied Biochemistry and Biotechnology, 2019, 187: 1255–1271.（唯一第一作者）

[8] Xue Cai, Hangyu Jiang, Tao Zhang*, Bo Jiang, Wanmeng Mu, Ming Miao. Thermostability and specific-activity enhancement of an arginine deiminase from Enterococcus faecalis SK23.001 via semirational design for l-citrulline production. Journal of Agricultural and Food Chemistry, 2018, 66(33): 8841–8850.（唯一第一作者）

[9] Xue Cai, Ines Seitl, Wanmeng Mu, Tao Zhang, Timo Stressler, Lutz Fischer*, Bo Jiang*. Combination of sequence-based and in silico screening to identify novel trehalose synthases. Enzyme and Microbial Technology, 2018, 115: 62–72.（唯一第一作者）

[10] Xue Cai, Ines Seitl, Wanmeng Mu, Tao Zhang, Timo Stressler, Lutz Fischer*, Bo Jiang*. Biotechnical production of trehalose through the trehalose synthase pathway: current status and future prospects. Applied Microbiology and Biotechnology, 2018, 102(7): 2965–2976. （唯一第一作者）

[11] Ji-Dong Shen, Xue Cai, Zhi-Qiang Liu*, Yu-Guo Zheng. High-throughput screening of signal peptide library with novel fluorescent probe. ChemBioChem, 2022, 23(12): e202100523.

[12] Feng Zhang, Xue Cai, Feng Cheng, Jia-Ming Yu, Bin Wang, Zhi-Qiang Liu*, Yu-Guo Zheng. Immobilization of sucrose isomerase from Erwinia sp. with graphene oxide and its application in synthesizing isomaltulose. Applied Biochemistry and Biotechnology, 2022, 194(2): 709–724.

[13] Ji-Dong Shen, Xue Cai, Zhi-Qiang Liu*, Yu-Guo Zheng. Nitrilase: a promising biocatalyst in industrial applications for green chemistry. Critical Reviews in Biotechnology, 2021, 41(1): 72–93. 

[14] Ji-Dong Shen, Xue Cai, Ming Wang, Zhi-Qiang Liu*, Yu-Guo Zheng. Proposed mechanism for post-translational self-modification of Co-NHase based on Co2+ diffusion limitation. Biotechnology Journal, 2021, 16(10): e2100103.

[15] Ji-Dong Shen, Xue Cai, Ye-Wen Ni, Li-Qun Jin, Zhi-Qiang Liu*, Yu-Guo Zheng. Structural insights into the thermostability mechanism of a nitrile hydratase from Caldalkalibacillus thermarum by comparative molecular dynamics simulation. Proteins, 2021, 89 (8): 978–987.

[16] Jiang-Wei Shen, Xue Cai, Bao-Juan Dou, Feng-Yu Qi, Xiao-Jian Zhang, Zhi-Qiang Liu*, Yu-Guo Zheng. Expression and characterization of a CALB-type lipase from Sporisorium reilianum SRZ2 and its potential in short-chain flavor ester synthesis. Frontiers of Chemical Science and Engineering, 2020, 14(5): 868–879. 

[17] Xue-Mei Kang, Xue Cai, Zi-Han Huang, Zhi-Qiang Liu*, Yu-Guo Zheng. Construction of a highly active secretory expression system in Bacillus subtilis of a recombinant amidase by promoter and signal peptide engineering. International Journal of Biological Macromolecules, 2020, 143: 833–841. 

[18] Xue-Mei Kang, Xue Cai, Zhi-Qiang Liu*, Yu-Guo Zheng. Secretory expression and characterization of a novel amidase from Kluyvera cryocrescens in Bacillus subtilis. Biotechnology Letters, 2020, 42: 2367–2377. 

[19] Xue-Mei Kang, Xue Cai, Zhi-Qiang Liu*, Yu-Guo Zheng. Identification and characterization of an amidase from Leclercia adecarboxylata for efficient biosynthesis of l-phosphinothricin. Bioresource Technology, 2019, 289: 121658.