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更新时间：2025.05.02
总访问量：10

高炬灿

| 博士 副研究员 请选择

单位：

职务： 朝晖特聘副研究员

研究方向：

办公地址： 莫干山校区生物楼A211

办公电话：

电子邮箱： jucangao@zjut.edu.cn

  • 个人简介

    高炬灿，男，汉族，1993年出生，浙江杭州人。毕业浙江大学化学工程与生物工程学院，近年在“Nature Synthesis”，“Current Opinion in Biotechnology”，“Biotechnology and Bioengineering”等期刊杂志发表论文十余篇。主要从事基于合成生物学原理和基因组编辑技术的人工细胞工厂创建研究。


  • 科研项目

    1. 国家自然科学基金-青年基金，2025-01至今，30万元，主持，在研。

    2. 中国博士后科学基金-面上资助，2023-06至2024-09， 8万元，主持，结题。


  • 科研成果

    1. Gao J, Zuo Y, Xiao F, Wang Y, Li D, Xu J, Ye C, Feng L, Jiang L, Liu T, et al.: Biosynthesis of catharanthine in engineered Pichia pastoris. Nature Synthesis 2023, 2: 231–242. 

    2. Gao J, Gou Y, Lian J: Reconstitution and optimization of complex plant natural product biosynthetic pathways in microbial expression systems. Current Opinion in Biotechnology 2024, 87: 103136.

    3. Gao J, Xu J, Zuo Y, Ye C, Jiang L, Feng L, Huang L, Xu Z, Lian J: Synthetic Biology Toolkit for Marker-Less Integration of Multigene Pathways into Pichia pastoris via CRISPR/Cas9. ACS Synthetic Biology 2022, 11:623-633.

    4. Gao J, Ye C, Cheng J, Jiang L, Yuan X, Lian J: Enhancing Homologous Recombination Efficiency in Pichia pastoris for Multiplex Genome Integration Using Short Homology Arms. ACS Synthetic Biology 2022, 11:547-553.

    5. Gao J, Jiang L, Lian J: Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products. Synthetic and Systems Biotechnology 2021, 6:110-119.

    6. Ye C, Hong H, Gao J, Li M, Gou Y, Gao D, Dong C, Huang L, Xu Z, Lian J: Characterization and Engineering of Peroxisome Targeting Sequences for Compartmentalization Engineering in Pichia pastoris. Biotechnology and Bioengineering 2024, 121:2091-2105. 

    7. Ye C, Li M, Gao J, Zuo Y, Xiao F, Jiang X, Cheng J, Huang L, Xu Z, Lian J: Metabolic Engineering of Pichia pastoris for Overproduction of Cis-trans nepetalactol. Metabolic engineering 2024, 84:83-94. 

    8. Gou Y, Li D, Zhao M, Li M, Zhang J, Zhou Y, Xiao F, Liu G, Ding H, Sun C, Ye C, Dong C, Gao J, et al.: Intein-mediated Temperature Control for Complete Biosynthesis of Sanguinarine and its Halogenated Derivatives in Yeast. Nature Communications 2024, 15:5238. 

    9. Jiang X, Li M, Wang Z, Ye C, Gao J, Ai X, Bao J, Cheng J, Lian J: CRISPR-mediated rDNA Integration and Fluorescence Screening for Pathway Optimization in Pichia pastoris. Chem & Bio Engineering 2024.  

    10. Cheng J, Zuo Y, Liu G, Li D, Gao J, Xiao F, Huang L, Xu Z, Lian J: Development of a Pichia pastoris cell factory for efficient production of germacrene A: a precursor of β-elemene. Bioresources and Bioprocessing 2023, 10:38.

    11. Feng L, Xu J, Ye C, Gao J, Huang L, Xu Z, Lian J: Metabolic Engineering of Pichia pastoris for the Production of Triacetic Acid Lactone. Journal of Fungi 2023, 9:494.

    12. Gao D, Liu T, Gao J, Xu J, Gou Y, Pan Y, Li D, Ye C, Pan R, Huang L, et al.: De Novo Biosynthesis of Vindoline and Catharanthine in Saccharomyces cerevisiae. BioDesign Research 2022, 2022:0002.

    13. Zuo Y, Xiao F, Gao J, Ye C, Jiang L, Dong C, Lian J: Establishing Komagataella phaffii as a Cell Factory for Efficient Production of Sesquiterpenoid alpha-Santalene. Journal Of Agricultural And Food Chemistry 2022, 70:8024-8031.

    14. Gu Y, Gao J, Cao M, Dong C, Lian J, Huang L, Cai J, Xu Z: Construction of a series of episomal plasmids and their application in the development of an efficient CRISPR/Cas9 system in Pichia pastoris. World J Microbiol Biotechnol 2019, 35:79.

    15. Sun W, Zuo Y, Yao Z, Gao J, Shao Z, Lian J: Recent Advances in Synthetic Biology Applications of Pichia Species. Synthetic Biology of Yeasts 2022:251–292.

    16. Gao J, Cheng J, Lian J: Multiplex Marker-Less Genome Integration in Pichia pastoris Using CRISPR/Cas9. Methods in Molecular Biology 2024, 2760:157-167.

  • 个人简介

    高炬灿，男，汉族，1993年出生，浙江杭州人。毕业浙江大学化学工程与生物工程学院，近年在“Nature Synthesis”，“Current Opinion in Biotechnology”，“Biotechnology and Bioengineering”等期刊杂志发表论文十余篇。主要从事基于合成生物学原理和基因组编辑技术的人工细胞工厂创建研究。


  • 科研项目

    1. 国家自然科学基金-青年基金，2025-01至今，30万元，主持，在研。

    2. 中国博士后科学基金-面上资助，2023-06至2024-09， 8万元，主持，结题。


  • 科研成果

    1. Gao J, Zuo Y, Xiao F, Wang Y, Li D, Xu J, Ye C, Feng L, Jiang L, Liu T, et al.: Biosynthesis of catharanthine in engineered Pichia pastoris. Nature Synthesis 2023, 2: 231–242. 

    2. Gao J, Gou Y, Lian J: Reconstitution and optimization of complex plant natural product biosynthetic pathways in microbial expression systems. Current Opinion in Biotechnology 2024, 87: 103136.

    3. Gao J, Xu J, Zuo Y, Ye C, Jiang L, Feng L, Huang L, Xu Z, Lian J: Synthetic Biology Toolkit for Marker-Less Integration of Multigene Pathways into Pichia pastoris via CRISPR/Cas9. ACS Synthetic Biology 2022, 11:623-633.

    4. Gao J, Ye C, Cheng J, Jiang L, Yuan X, Lian J: Enhancing Homologous Recombination Efficiency in Pichia pastoris for Multiplex Genome Integration Using Short Homology Arms. ACS Synthetic Biology 2022, 11:547-553.

    5. Gao J, Jiang L, Lian J: Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products. Synthetic and Systems Biotechnology 2021, 6:110-119.

    6. Ye C, Hong H, Gao J, Li M, Gou Y, Gao D, Dong C, Huang L, Xu Z, Lian J: Characterization and Engineering of Peroxisome Targeting Sequences for Compartmentalization Engineering in Pichia pastoris. Biotechnology and Bioengineering 2024, 121:2091-2105. 

    7. Ye C, Li M, Gao J, Zuo Y, Xiao F, Jiang X, Cheng J, Huang L, Xu Z, Lian J: Metabolic Engineering of Pichia pastoris for Overproduction of Cis-trans nepetalactol. Metabolic engineering 2024, 84:83-94. 

    8. Gou Y, Li D, Zhao M, Li M, Zhang J, Zhou Y, Xiao F, Liu G, Ding H, Sun C, Ye C, Dong C, Gao J, et al.: Intein-mediated Temperature Control for Complete Biosynthesis of Sanguinarine and its Halogenated Derivatives in Yeast. Nature Communications 2024, 15:5238. 

    9. Jiang X, Li M, Wang Z, Ye C, Gao J, Ai X, Bao J, Cheng J, Lian J: CRISPR-mediated rDNA Integration and Fluorescence Screening for Pathway Optimization in Pichia pastoris. Chem & Bio Engineering 2024.  

    10. Cheng J, Zuo Y, Liu G, Li D, Gao J, Xiao F, Huang L, Xu Z, Lian J: Development of a Pichia pastoris cell factory for efficient production of germacrene A: a precursor of β-elemene. Bioresources and Bioprocessing 2023, 10:38.

    11. Feng L, Xu J, Ye C, Gao J, Huang L, Xu Z, Lian J: Metabolic Engineering of Pichia pastoris for the Production of Triacetic Acid Lactone. Journal of Fungi 2023, 9:494.

    12. Gao D, Liu T, Gao J, Xu J, Gou Y, Pan Y, Li D, Ye C, Pan R, Huang L, et al.: De Novo Biosynthesis of Vindoline and Catharanthine in Saccharomyces cerevisiae. BioDesign Research 2022, 2022:0002.

    13. Zuo Y, Xiao F, Gao J, Ye C, Jiang L, Dong C, Lian J: Establishing Komagataella phaffii as a Cell Factory for Efficient Production of Sesquiterpenoid alpha-Santalene. Journal Of Agricultural And Food Chemistry 2022, 70:8024-8031.

    14. Gu Y, Gao J, Cao M, Dong C, Lian J, Huang L, Cai J, Xu Z: Construction of a series of episomal plasmids and their application in the development of an efficient CRISPR/Cas9 system in Pichia pastoris. World J Microbiol Biotechnol 2019, 35:79.

    15. Sun W, Zuo Y, Yao Z, Gao J, Shao Z, Lian J: Recent Advances in Synthetic Biology Applications of Pichia Species. Synthetic Biology of Yeasts 2022:251–292.

    16. Gao J, Cheng J, Lian J: Multiplex Marker-Less Genome Integration in Pichia pastoris Using CRISPR/Cas9. Methods in Molecular Biology 2024, 2760:157-167.

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更新时间：2025.05.02
总访问量：10

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