黄金

学习及工作简历：

• 1998年-2002年    安徽科技学院        食品工程                                  本科；

• 2003年-2008年    天津科技大学        发酵工程                           硕博连读；

• 2008年-2011年    浙江大学               生物化工                              博士后；

• 2011年-2013年    浙江工业大学        微生物与生化药学                    讲师；

• 2013年-                浙江工业大学        微生物与生化药学                副教授；

• 2015年-2016年    俄亥俄州立大学     化学与生物分子工程系      访问学者；

主辅讲课程：

本科生：

• 《生物合成药物学》；

• 《微生物制药学实验》；

• 《生物制药专业认识实习》；

• 《生物制药专业生产实习》；

研究生：

• 《生物催化技术》；

• 《生物制药技术》；

育人成果

• 浙江工业大学优秀班主任（2014、2019）；

• 浙江工业大学优秀本科生实习指导教师（2019）；

• 浙江工业大学药学院优秀班主任（2013、2019）；

• 浙江工业大学药学院说课竞赛二等奖（2013）；
  

主持科研项目及教改项目

纵向：

• 国家自然科学基金项目          21406204；

• 浙江省科技厅项目             2014C33274；

• 浙江省自然科学基金项目         LQ12B06005；

• 浙江省教育厅一般项目          Y201226056；

横向：

• 新型代谢标记物的应用                KYY-HX-20170528；    

• 有机酸提纯新工艺开发                KYY-HX-20190343；

• 生物炼制与催化校企科研基金            2012040;

• 天然护肤因子直接发酵法生产工艺研究  KYY-HX-20230315；

教改：

• 浙江工业大学教学改革项目         JG1250；

• 浙江工业大学教学改革项目         JG201717；

• 浙江工业大学重点建设教材项目      JC1720；

学术论文

43.C.Zhong, L. Liu, W. Ding, H. Lin, J. Huang# (2025). .Exploration of novel salt-tolerant β-galactosidases for GOS synthesis  based on whole genome sequencing of Bacillus hwajinpoensis Zjut  HJ2101. Biotechnology and Bioprocess Engineering , https://doi.org/10.1007/s12257-025-00231-9（JCR Q2）

42.C.Zhong, W. Ding, H. Lin, J. Huang# (2025). .Improved biosynthesis of lactulose by a novel thermostable cellobiose-2-epimerase generated through semi-rational mutagenesis. World Journal of Microbiology and Biotechnology, 41:451. https://doi.org/10.1007/s11274-025-04683-2（JCR Q2）

41.J. Duan, M. Zhu, X. Ma, L. Liu, J. Huang# (2025). Solubility, metastable zone, and cooling crystallization process of dodecanedioic acid investigated by Focused Beam Reflectance Measurement (FBRM) technology. Journal of Chemical Technology and Biotechnology,100:1482-1490.  https://doi.org/10.1002/jctb.7881.（JCR Q2）

40.L.Jin, H. Lin, G. Wang, X. Wang, J. Huang# (2025). Construction of dynamic down-regulation systems in Clostridium tyrobutyricum for butyrate production. Process Biochemistry, 152: 47-57.https://doi.org/10.1016/j.procbio.2025.02.013.（JCR Q1）

39.G. Sun, L. Jin, G. Wang, X. Wang, J. Huang# (2024). Expression of ATP-binding cassette transporter proteins AatA or MdlB facilitates butyric acid production in Clostridium tyrobutyricum, Biochemical Engineering Journal, 201:109142.https://doi.org/10.1016/j.bej.2023.109142（JCR Q2）

38.G. Wang, J. Jiang, L., J. Huang# (2024). Recombinant beta-galactosidase derived from Enterobacter cloacae Zjut HJ2001 for efficient biotransformation of galactooligosaccharides. Biochemical Engineering Journal, 212:109514. https://doi.org/10.1016/j.bej.2024.109514.（JCR Q2）

37.J. Duan, L. Chen, R. Hong, Y. Li, J. Huang# (2023). Efficient crystallization process of dodecanedioic acid by a pneumatically agitated crystallizer. Preparative Biochemistry & Biotechnology. https ://doi.org/10.1080/10826068.2022.2164593. （JCR Q2）

36.L. Zhao, G. Sun, J. Jiang, L. Chen, J. Huang# (2021). Role of microbubbles coupling fibrous-bed bioreactor in butyric acid production by Clostridium tyrobutyricum using Brewer’s spent grain as feedstock, Biochemical Engineering Journal, 172:108051. https://doi.org/10.1016/j.bej.2021.108051.（JCR Q2）

35.J. Huang#, S. Zhu, L. Zhao, L. Chen, M. Du, C. Zhang, S. Yang (2020). A novel β-galactosidase from Klebsiella oxytoca ZJUH1705 for efficient production of galacto-oligosaccharides from lactose, Appl. Microbiol. Biotechnol, 104: 161-6172. https://doi.org/10.1007/s00253-020-10679-9.（JCR Q1）

34.J. Huang, Y. Du, T. Bao, M. Lin, J. Wang, S. Yang# (2019).  Production of n-butanol from cassava bagasse hydrolysate by engineered Clostridium tyrobutyricum overexpressing adhE2: Kinetics and cost analysis, Bioresource Technology, 292: 121969. https://doi.org/10.1016/j.biortech.2019.121969.（JCR Q1）

33.J. Huang#, W. Tang, S. Zhu, M. Du (2018). Biosynthesis of butyric acid by Clostridium tyrobutyricum, Preparative Biochemistry & Biotechnology, 48: 427-434. https://doi.org/10.1080/10826068.2018.1452257.（JCR Q2）

32.J. Huang#, W. Tang, S. Zhu, M. Du (2018). Production of butyric acid by Clostridium tyrobutyricum coated with carboxyl functioned magnetic nanoparticles, Journal of Chemical Technology and Biotechnology, 93: 2364-2370. https://doi.org/10.1002/jctb.5582.（JCR Q1）

31.J. Huang#, H. Zhu, W. Tang, P. Wang, S. Yang (2016). Butyric acid production from oilseed rape straw by Clostridium tyrobutyricum immobilized in a fibrous bed bioreactor, Process Biochemistry, 51: 1930-1934. https://doi.org/10.1016/j.procbio.2016.08.019.（JCR Q1）

30.J. Huang, R. Yan, J. He, P. Wang# (2016). Purification and immobilization of a novel enantioselective lipase from Tsukamurella tyrosinosolvents for efficient fesolution of ethyl 2-(2-oxopyrrolidin-1-yl) butyrate. Appl. Biochem. Biotechnol, 180: 609-622. https://doi.org/10.1007/s12010-016-2119-3.（JCR Q2）

29.J. Huang#, S. Zhu, C. Li, C. Zhang, Y. Ji (2020). Cost-effective optimization of gellan gum production by Sphingomonas paucimobilis using corn steep liquor, Preparative Biochemistry & Biotechnology, 50: 191-197. https://doi.org/10.1080/10826068.2019.1692215.（JCR Q2）

28.J. Huang#, H. Dai, R. Yan, P. Wang (2016). Butyric acid production from recycled waste paper by immobilized Clostridium tyrobutyricum in a fibrous-bed bioreactor, Journal of Chemical Technology and Biotechnology, 91: 1048-1054. https://doi.org/10.1002/jctb.4680.（JCR Q1）

27.J. Huang, R.Yan, H.Shi, P. Wang#, Junyao He (2014). The characteristic and kinetic studies on the enantioselective hydrolysis of (R, S)-α-ethyl-2-oxo-1-pyrrolidine acetic acid ethyl ester catalyzed by Tsukamurella tyrosinosolvens E105-derived hydrolase, Asia-Pac. J. Chem. Eng., 9: 941-949. https://doi.org/10.1002/apj.1848.（JCR Q3）

26.J. Huang, S. Xie, J. He, P. Wang# (2012). Effects of ionic liquids on the growth of Arthrobacter simplex and improved biodehydrogenation in an ionic liquid-containing system with immobilized cells. Appl. Biochem. Biotechnol, 167: 2131-2143.  https://doi.org/10.1007/s12010-012-9762-0.（JCR Q2）

25.J. Huang, S. Jiang, X. Xu, H. Wu, X.g Zhu, Z. Ke, J. Cai, L.Huang, Z. Xu# (2012). Effects of carbon/nitrogen ratio, dissolved oxygen and impeller type on gellan gum production in Sphingomonas paucimobilis, Ann. Microbiol. 62: 299-305. https://doi.org/10.1007/s13213-011-0261-2.（JCR Q2）

24.J. Huang, Y. Du,G. Xu,H. Zhang,F. Zhu,L. Huang,Z. Xu#(2011). High yield and cost-effective production of poly(γ-glutamic acid) with Bacillus subtilis. Eng. Life Sci., 11: 291-297. https://doi.org/10.1002/elsc.201000133.（JCR Q2）

23.J. Huang#, H. Dai, R. Yan, P. Wang (2016). Enhanced production of butyric acid through immobilization of Clostridium tyrobutyricum in a novel inner disc-shaped matrix bioreactor, Ann Microbiol,66,121-129. https://doi.org/10.1007/s13213-015-1088-z.（JCR Q2）

22.J. Huang, J. Cai, J. Wang, X. Zhu, L.Huang, S. Yang, Z. Xu# (2011). Efficient  production  of butyric acid from Jerusalem artichoke by immobilized Clostridium tyrobutyricum in a fibrous-bed bioreactor, Bioresource Technology, 102: 3923-3926. https://doi.org/10.1016/j.biortech.2010.11.112.（JCR Q1）

21.J. Huang, L. Huang, J. Lin, Z. Xu, P. Cen# (2010). Organic chemicals from bioprocesses in China. In: Tsao, G., Ouyang, P., Chen, J. (eds) Biotechnology in China II, Advances in Biochemical Engineering/Biotechnology, vol 122. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2010_75.（JCR Q2）

20. J. Huang, Q. Xu, T. Wen, N. Chen# (2008) Metabolic flux analysis of L-threonine biosynthesis strain under diverse dissolved oxygen conditions. Acta Microbiology Sinica, 48(8):1056-1060.

19.F. Liang, J. Huang#, J. He, P. Wang# (2012).Improved enantioselective hydrolysis of racemic ethyl-2,2-dimethylcyclopropanecarboxylate catalyzed by modified Novozyme 435, Biotechnol. Bioproc. E, 17, 952-958. https://doi.org/10.1007/s12257-012-0135-x.（JCR Q2）

18.Q. Ouyang, P. Wang#, J. Huang#, J. Cai, J. He (2013). Efficient enantioselective synthesis of (R)-[3,5-bis(trifluoromethyl)phenyl] ethanol by Leifsonia xyli CCTCC M 2010241 using isopropanol as co-substrate, Journal of microbiology and biotechnology, 23: 343-350. https://doi.org/10.4014/jmb.1203.03047.（JCR Q2）

17.W. Luo, J. Huang, X. Zhu, L. Huang, J. Cai, Z. Xu# (2013). Enhanced production of L-tryptophan with glucose feeding and surfactant addition and related metabolic flux redistribution in the recombinant Escherichia coli, Food Sci. Biotechnol, 22: 207-214. https://doi.org/10.1007/s10068-013-0029-5.（JCR Q2）

16.F. Zhu, J. Cai, X. Wu, J. Huang, L. Huang, J. Zhu, Q. Zheng, P. Cen, Z. Xu# (2013). The main byproducts and metabolic flux profiling of γ-PGA-producing strain B. subtilis ZJU-7 under different pH values, Journal of Biotechnology, 164:67-74. https://doi.org/10.1016/j.jbiotec.2012.12.009.（JCR Q1）

15.J. Li, P. Wang#, J.He, J. Huang, J. Tang (2013). Efficient biocatalytic synthesis of (R)-[3,5-bis(trifluoromethyl)phenyl] ethanol by a newly isolated Trichoderma asperellum ZJPH0810 using dual cosubstrate: ethanol and glycerol, Appl. Microbiol. Biotechnol, 97: 6685-6692. https://doi.org/10.1007/s00253-013-4973-z.（JCR Q1）

14.J. Li, P.Wang#, Y. He, Z. Zhu, and J. Huang (2019). Toward designing a novel oligopeptide-based deep eutectic solvent: applied in biocatalytic reduction, ACS Sustainable Chemistry & Engineering, 7:1318-1326. https://doi.org/10.1021/acssuschemeng.8b04989.（JCR Q1）

13.N. Wang, J. Li, J. Sun, J.Huang, P. Wang #(2015). Bioreduction of 3,5-bis(trifluoromethyl)acetophenone using ionic liquid as a co-solvent catalyzed by recombinant Escherichia coli cells, Biochemical Engineering Journal, 101: 119-125. https://doi.org/10.1016/j.bej.2015.05.013.（JCR Q2）

12.H. Zhang, J. Zhu, X. Zhu, J. Cai, A. Zhang, Y. Hong, J. Huang, L. Huang, Z. Xu #(2012). High-level exogenous glutamic acid-independent production of poly-(γ-glutamic acid) with organic acid addition in a new isolated Bacillus subtilis C10, Bioresource Technology, 116: 241-246. https://doi.org/10.1016/j.biortech.2011.11.085.（JCR Q1）

11.W.Zhang, J. Huang, X. Wo, P. Wang# (2013). Microbial transformation of curcumin to its derivatives with a novel Pichia kudriavzevii ZJPH0802 strain, Appl. Biochem. Biotechnol, 170: 1026-1037. https://doi.org/10.1007/s12010-013-0256-5.（JCR Q2）

10.J. Li, P. Wang#, J. Huang, J. Sun (2015). Design and application of a novel ionic liquid with the property of strengthening coenzyme regeneration for whole-cell bioreduction in an ionic liquid-distilled water medium, Bioresource Technology, 175: 42-50. https://doi.org/10.1016/j.biortech.2014.10.059.（JCR Q1）

9.W. Luo, P. Wei, H. Chen, L. Fan, L. Huang, J. Huang, Z. Xu# & P. Cen(2011). Kinetics and optimization of L-tryptophan separation with ion-exchange chromatography. Korean J. Chem. Eng. 28: 1280-1285.https://doi.org/10.1007/s11814-010-0489-8.（JCR Q2）

8.W. Luo, H. Chen, L. Fan, J. Huang, L. Huang, Z. Xu# & P. Cen(2011). Adsorption behavior of L-tryptophan on ion exchange resin, Korean J. Chem. Eng. 28, 1272-1279. https://doi.org/10.1007/s11814-010-0490-2.（JCR Q2）

7.J. Sun, J.Huang, X. Ding & P. Wang #(2016). Efficient enantioselective biocatalytic production of a chiral intermediate of sitagliptin by a newly filamentous fungus isolate, Appl. Biochem. Biotechnol, 180, 695-706. https://doi.org/10.1007/s12010-016-2125-5.（JCR Q2）

6.N. Wang, J. Sun, J. Huang, P. Wang# (2014). Cloning, expression, and directed evolution of carbonyl reductase from Leifsonia xyli HS0904 with enhanced catalytic efficiency, Appl. Microbiol. Biotechnol, 98: 8591-8601. https://doi.org/10.1007/s00253-014-5770-z.（JCR Q1）

5.N. Chen#, J. Huang, Z. Feng, L. Yu, Q. Xu, T. Wen(2009). Optimization of fermentation conditions for the biosynthesis of L-Threonine by Escherichia coli, Appl Biochem Biotechnol 158: 595-604. https://doi.org/10.1007/s12010-008-8385-y.（JCR Q2）

4.D. Bai, J. He, B. Ouyang, J. Huang, P. Wang# (2017). Biocatalytic asymmetric synthesis of chiral aryl alcohols. Progress in Chemistry, 29: 491-501.https://doi.org/10.7536/PC170222.（JCR Q3）

3.F. Ren, P. Wang#, J. Huang, J. He (2012). Enzymatic resolution of racemic ethyl-2,2-dimethylcyclopropanecarboxylate to S-(+)-2,2-dimethylcyclopropanecarboxylic acid in a polar organic solvent-water medium, Biotechnology & Biotechnological Equipment, 26: 3412-3417.https://doi.org/10.5504/BBEQ.2012.0109（JCR Q3）

2.N. Wang, J. Huang, H. Luo, P. Wang#, J. Li (2013). Purification and characterization of a new carbonyl reductase from Leifsonia xyli HS0904 involved in stereoselective reduction of 3,5-bis(trifluoromethyl) acetophenone, Journal of Molecular Catalysis B: Enzymatic, 92:1-6. https://doi.org/10.1016/j.molcatb.2013.03.007.（JCR Q2）

1.Z. Xu#, H. Zhang, H. Chen, F. Shi, J. Huang, S. Wang, C. Song (2013). Microbial Production of Poly-γ-Glutamic Acid. In Bioprocessing Technologies in Biorefinery for Sustainable Production of Fuels, Chemicals, and Polymers (eds S.-T. Yang, H.A. El-Enshasy and N. Thongchul). https://doi.org/10.1002/9781118642047.ch23.

发明专利及获奖

10. 黄金, 丁卫健, 等. 一种耐高温纤维二糖差向异构酶突变体及其应用, 20250901, ZL202411681374.4;

9.   黄金, 洪锐, 等. 一种基于随机森林筛选高活性色氨酸酶的方法及应用, 20250818, ZL202411936314.2;

8.  黄金, 王广雪, 等. 花津滩芽孢杆菌Zjut HJ2101及其在生产低聚半乳糖中的应用, 20250509, ZL202210915746.X;

7.   黄金, 陈李, 等. 一种十二碳二元酸晶种的制备方法及其应用, 20241122, ZL202210663486.8;

6.   黄金, 竺胜权, 等. 产酸克雷伯菌及其应用, 20201225, ZL201711112168.1;

5.   黄金,姜静, 等. 阴沟肠杆菌ZjutHJ2001及其在发酵产低聚半乳糖中的应用,20240326,ZL202210691233.5;

4.   黄金, 王邵冰, 等. 一种长链二元酸精制装置的排料系统, 20220107, ZL202120319563.2;

3.   黄金, 姜静, 等. 一种乳糖不耐受症检测试剂盒, 20240116, ZL202321242278.0;

2.   黄金, 王邵冰,  等.  一种长链二元酸精制装置, 20211210, ZL202120321526.5;

1.   黄金(4/9); 西司他丁重要手性中间体的绿色合成关键技术研究及产业化开发, 浙江省科技厅, 科技进步, 省部二等奖, 2017;

行业标准

1.《二十二碳六烯酸油脂粉》  QB/T 5632-2021;

2.《花生四烯酸微胶囊》     QB/T 5631-2021;

3.《D-乳酸》                        QB/T 5937-2023;

其他

1.担任《Bioresource Technology》、《Applied Microbiology and Biotechnology》、《Journal of Chemical Technology and Biotechnology》、《Microbial Cell Factories》、《Process Biochemistry》、《Applied Biochemistry and Biotechnology》、《Engineering in Life Science》等SCI期刊国际审稿人，获得2018年度《Process Biochemistry》Outstanding Reviewer；

2. 国家自然科学基金、广东省自然科学基金、广东省科技厅项目、教育部学位中心学位论文等评审专家；

3. 主编教材《工业微生物实验技术教程》、参编《工业微生物分子生物学实验原理与技术》；