黄金

学习及工作简历：

• 1998年-2002年    安徽科技学院        食品工程                                  本科；

• 2003年-2008年    天津科技大学        发酵工程                           硕博连读；

• 2008年-2011年    浙江大学               生物化工                              博士后；

• 2011年-2013年    浙江工业大学        微生物与生化药学                    讲师；

• 2013年-                浙江工业大学        微生物与生化药学                副教授；

• 2015年-2016年    俄亥俄州立大学     化学与生物分子工程系      访问学者；

主辅讲课程：

本科生：

• 《生物合成药物学》；

• 《微生物制药学实验》；

• 《生物制药专业认识实习》；

• 《生物制药专业生产实习》；

研究生：

• 《生物催化技术》；

• 《生物制药技术》；

育人成果

• 浙江工业大学优秀班主任（2014、2019）；

• 浙江工业大学优秀本科生实习指导教师（2019）；

• 浙江工业大学药学院优秀班主任（2013、2019）；

• 浙江工业大学药学院说课竞赛二等奖（2013）；
  

主持科研项目及教改项目

纵向：

• 国家自然科学基金项目          21406204；

• 浙江省科技厅项目             2014C33274；

• 浙江省自然科学基金项目         LQ12B06005；

• 浙江省教育厅一般项目          Y201226056；

横向：

• 新型代谢标记物的应用                KYY-HX-20170528；    

• 有机酸提纯新工艺开发                KYY-HX-20190343；

• 生物炼制与催化校企科研基金            2012040;

• 天然护肤因子直接发酵法生产工艺研究  KYY-HX-20230315；

教改：

• 浙江工业大学教学改革项目         JG1250；

• 浙江工业大学教学改革项目         JG201717；

• 浙江工业大学重点建设教材项目      JC1720；

学术论文

1.Gaoya Sun, Lingkai Jin, Guangxue Wang, Xiaoge Wang, Jin Huang# (2024). Expression of ATP-binding cassette transporter proteins AatA or MdlB facilitates butyric acid production in Clostridium tyrobutyricum, Biochemical Engineering Journal, 201:109142.

2.Linqi Zhao, Gaoya Sun, Jing Jiang, Li Chen, Jin Huang# (2021). Role of microbubbles coupling fibrous-bed bioreactor in butyric acid production by Clostridium tyrobutyricum using Brewer’s spent grain as feedstock, Biochemical Engineering Journal, 172:108051. https://doi.org/10.1016/j.bej.2021.108051.

3.Jin Huang#, Shengquan Zhu, Linqi Zhao, Li Chen, Meini Du, Chenglin Zhang, Shang-Tian Yang (2020). A novel β-galactosidase from Klebsiella oxytoca ZJUH1705 for efficient production of galacto-oligosaccharides from lactose, Appl. Microbiol. Biotechnol, 104: 6161-6172. https://doi.org/10.1007/s00253-020-10679-9.

4.Jin Huang#, Shengquan Zhu, Chuanbao Li, Chenglin Zhang & Yizhi Ji (2020). Cost-effective optimization of gellan gum production by Sphingomonas paucimobilis using corn steep liquor, Preparative Biochemistry & Biotechnology, 50: 191-197. https://doi.org/10.1080/10826068.2019.1692215.

5.Jin Huang, Yinming Du, Teng Bao, Meng Lin, Jufang Wang, Shang-Tian Yang# (2019).  Production of n-butanol from cassava bagasse hydrolysate by engineered Clostridium tyrobutyricum overexpressing adhE2: Kinetics and cost analysis, Bioresource Technology, 292: 121969. https://doi.org/10.1016/j.biortech.2019.121969.

6.Jin Huang#, Han Zhu, Wan Tang, Pu Wang, Shang-Tian Yang (2016). Butyric acid production from oilseed rape straw by Clostridium tyrobutyricum immobilized in a fibrous bed bioreactor, Process Biochemistry, 51: 1930-1934. https://doi.org/10.1016/j.procbio.2016.08.019.

7.Jin Huang#, Wan Tang, Shengquan Zhu & Meini Du (2018). Biosynthesis of butyric acid by Clostridium tyrobutyricum, Preparative Biochemistry & Biotechnology, 48: 427-434. https://doi.org/10.1080/10826068.2018.1452257.

8.Jin Huang#, Wan Tang, Shengquan Zhu, Meini Du (2018). Production of butyric acid by Clostridium tyrobutyricum coated with carboxyl functioned magnetic nanoparticles, Journal of Chemical Technology and Biotechnology, 93: 2364-2370. https://doi.org/10.1002/jctb.5582.

9.Jin Huang, Ren Yan, Haifang Shi, Pu Wang#, Junyao He (2014). The characteristic and kinetic studies on the enantioselective hydrolysis of (R, S)-α-ethyl-2-oxo-1-pyrrolidine acetic acid ethyl ester catalyzed by Tsukamurella tyrosinosolvens E105-derived hydrolase, Asia-Pac. J. Chem. Eng., 9: 941-949. https://doi.org/10.1002/apj.1848.

10.Jin Huang, Ren Yan, Jun-yao He & Pu Wang# (2016). Purification and immobilization of a novel enantioselective lipase from Tsukamurella tyrosinosolvents for efficient fesolution of ethyl 2-(2-oxopyrrolidin-1-yl) butyrate. Appl. Biochem. Biotechnol, 180: 609-622. https://doi.org/10.1007/s12010-016-2119-3.

11.Jin Huang#, Hongliang Dai, Ren Yan & Pu Wang (2016). Enhanced production of butyric acid through immobilization of Clostridium tyrobutyricum in a novel inner disc-shaped matrix bioreactor, Ann Microbiol,66,121-129. https://doi.org/10.1007/s13213-015-1088-z.

12.Jin Huang#, Hongliang Dai, Ren Yan, Pu Wang (2016). Butyric acid production from recycled waste paper by immobilized Clostridium tyrobutyricum in a fibrous-bed bioreactor, Journal of Chemical Technology and Biotechnology, 91: 1048-1054. https://doi.org/10.1002/jctb.4680.

13.Jin Huang, Songlin Xie, Junyao He, Pu Wang# (2012). Effects of ionic liquids on the growth of Arthrobacter simplex and improved biodehydrogenation in an ionic liquid-containing system with immobilized cells. Appl. Biochem. Biotechnol, 167: 2131-2143.  https://doi.org/10.1007/s12010-012-9762-0.

14.Jin Huang, Shu Jiang, Xiaoqin Xu, Haishui Wu, Xiangcheng Zhu, Zuming Ke, Jin Cai, Lei Huang & Zhinan Xu# (2012). Effects of carbon/nitrogen ratio, dissolved oxygen and impeller type on gellan gum production in Sphingomonas paucimobilis, Ann. Microbiol. 62: 299-305. https://doi.org/10.1007/s13213-011-0261-2.

15.Jin Huang, Yinming Du,Guohua Xu,Huili Zhang,Fan Zhu,Lei Huang,Zhinan Xu#(2011). High yield and cost-effective production of poly(γ-glutamic acid) with Bacillus subtilis. Eng. Life Sci., 11: 291-297. https://doi.org/10.1002/elsc.201000133.

16.Jin Huang, Jin Cai, Jin Wang, Xiangcheng Zhu, Lei Huang, Shang-Tian Yang, Zhinan Xu# (2011). Efficient  production  of butyric acid from Jerusalem artichoke by immobilized Clostridium tyrobutyricum in a fibrous-bed bioreactor, Bioresource Technology, 102: 3923-3926. https://doi.org/10.1016/j.biortech.2010.11.112.

17.Jin Huang, Lei Huang, Jianping Lin, Zhinan Xu, Peilin Cen# (2010). Organic chemicals from bioprocesses in China. In: Tsao, G., Ouyang, P., Chen, J. (eds) Biotechnology in China II, Advances in Biochemical Engineering/Biotechnology, vol 122. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2010_75.

18.Jiulong Duan, Li Chen, Rui Hong, Yanjun Li, Jin Huang# (2023).  Efficient crystallization process of dodecanedioic acid by a pneumatically agitated crystallizer. Preparative Biochemistry & Biotechnology. https ://doi.org/10.1080/10826068.2022.2164593.

19.Gaoya Sun, Lingkai Jin, Guangxue Wang, Xiaoge Wang, Jin Huang# (2024). Expression of ATP-binding cassette transporter proteins AatA or MdlB facilitates butyric acid production in Clostridium tyrobutyricum. Biochemical Engineering Journal, 201:109142. https://doi.org/10.1016/j.bej.2023.109142

20.Jin Huang, Qingyang Xu, Tingyi Wen, Ning Chen# (2008) Metabolic flux analysis of L-threonine biosynthesis strain under diverse dissolved oxygen conditions. Acta Microbiology Sinica, 48(8):1056-1060.

21.Fa-yong Liang, Jin Huang#, Jun-yao He, Pu Wang# (2012).Improved enantioselective hydrolysis of racemic ethyl-2,2-dimethylcyclopropanecarboxylate catalyzed by modified Novozyme 435, Biotechnol. Bioproc. E, 17, 952-958. https://doi.org/10.1007/s12257-012-0135-x.

22.Qi Ouyang, Pu Wang#, Jin Huang#, Jinbo Cai, Junyao He (2013). Efficient enantioselective synthesis of (R)-[3,5-bis(trifluoromethyl)phenyl] ethanol by Leifsonia xyli CCTCC M 2010241 using isopropanol as co-substrate, Journal of microbiology and biotechnology, 23: 343-350. https://doi.org/10.4014/jmb.1203.03047.

23.Weiyu Zhang, Jin Huang, Xingde Wo, Pu Wang# (2013). Microbial transformation of curcumin to its derivatives with a novel Pichia kudriavzevii ZJPH0802 strain, Appl. Biochem. Biotechnol, 170: 1026-1037. https://doi.org/10.1007/s12010-013-0256-5.

24.Wei Luo, Jin Huang, Xiangcheng Zhu, Lei Huang, Jin Cai, Zhinan Xu# (2013). Enhanced production of L-tryptophan with glucose feeding and surfactant addition and related metabolic flux redistribution in the recombinant Escherichia coli, Food Sci. Biotechnol, 22: 207-214. https://doi.org/10.1007/s10068-013-0029-5.

25.Fan Zhu, Jin Cai, Xiangting Wu, Jin Huang, Lei Huang, Jianzhong Zhu, Qiang Zheng, Peilin Cen, Zhinan Xu# (2013). The main byproducts and metabolic flux profiling of γ-PGA-producing strain B. subtilis ZJU-7 under different pH values, Journal of Biotechnology, 164:67-74. https://doi.org/10.1016/j.jbiotec.2012.12.009.

26.Jun Li, Pu Wang#, Jun-Yao He, Jin Huang, Jun Tang (2013). Efficient biocatalytic synthesis of (R)-[3,5-bis(trifluoromethyl)phenyl] ethanol by a newly isolated Trichoderma asperellum ZJPH0810 using dual cosubstrate: ethanol and glycerol, Appl. Microbiol. Biotechnol, 97: 6685-6692. https://doi.org/10.1007/s00253-013-4973-z.

27.Jun Li, Pu Wang#, Yu-Shu He, Zhi-Ren Zhu, and Jin Huang (2019). Toward designing a novel oligopeptide-based deep eutectic solvent: applied in biocatalytic reduction, ACS Sustainable Chemistry & Engineering, 7:1318-1326. https://doi.org/10.1021/acssuschemeng.8b04989.

28.Nengqiang Wang, Jun Li, Jing Sun, Jin Huang, Pu Wang #(2015). Bioreduction of 3,5-bis(trifluoromethyl)acetophenone using ionic liquid as a co-solvent catalyzed by recombinant Escherichia coli cells, Biochemical Engineering Journal, 101: 119-125. https://doi.org/10.1016/j.bej.2015.05.013.

29.Huili Zhang, Jianzhong Zhu, Xiangcheng Zhu, Jin Cai, Anyi Zhang, Yizhi Hong, Jin Huang, Lei Huang, Zhinan Xu #(2012). High-level exogenous glutamic acid-independent production of poly-(γ-glutamic acid) with organic acid addition in a new isolated Bacillus subtilis C10, Bioresource Technology, 116: 241-246. https://doi.org/10.1016/j.biortech.2011.11.085.

30.Jun Li, Pu Wang#, Jin Huang, Jia Sun (2015). Design and application of a novel ionic liquid with the property of strengthening coenzyme regeneration for whole-cell bioreduction in an ionic liquid-distilled water medium, Bioresource Technology, 175: 42-50. https://doi.org/10.1016/j.biortech.2014.10.059.

31.Wei Luo, Peilian Wei, Hao Chen, Limei Fan, Lei Huang, Jin Huang, Zhinan Xu# & Peilin Cen(2011). Kinetics and optimization of L-tryptophan separation with ion-exchange chromatography. Korean J. Chem. Eng. 28: 1280-1285.https://doi.org/10.1007/s11814-010-0489-8.

32.Wei Luo, Hao Chen, Limei Fan, Jin Huang, Lei Huang, Zhinan Xu# & Peilin Cen(2011). Adsorption behavior of L-tryptophan on ion exchange resin, Korean J. Chem. Eng. 28, 1272-1279. https://doi.org/10.1007/s11814-010-0490-2.

33.Jia Sun, Jin Huang, Xuzhong Ding & Pu Wang #(2016). Efficient enantioselective biocatalytic production of a chiral intermediate of sitagliptin by a newly filamentous fungus isolate, Appl. Biochem. Biotechnol, 180, 695-706. https://doi.org/10.1007/s12010-016-2125-5.

34.Neng-Qiang Wang, Jing Sun, Jin Huang, Pu Wang# (2014). Cloning, expression, and directed evolution of carbonyl reductase from Leifsonia xyli HS0904 with enhanced catalytic efficiency, Appl. Microbiol. Biotechnol, 98: 8591-8601. https://doi.org/10.1007/s00253-014-5770-z.

35.Ning Chen#, Jin Huang, Zhi-bin Feng, Lei Yu, Qing-yang Xu, Ting-yi Wen(2009). Optimization of fermentation conditions for the biosynthesis of L-Threonine by Escherichia coli, Appl Biochem Biotechnol 158: 595-604. https://doi.org/10.1007/s12010-008-8385-y.

36.Dongya Bai, Junyao He, Bin Ouyang, Jin Huang, Pu Wang# (2017). Biocatalytic asymmetric synthesis of chiral aryl alcohols. Progress in Chemistry, 29: 491-501.https://doi.org/10.7536/PC170222.

37.Feng Ren, Pu Wang#, Jin Huang, Junyao He (2012). Enzymatic resolution of racemic ethyl-2,2-dimethylcyclopropanecarboxylate to S-(+)-2,2-dimethylcyclopropanecarboxylic acid in a polar organic solvent-water medium, Biotechnology & Biotechnological Equipment, 26: 3412-3417.https://doi.org/10.5504/BBEQ.2012.0109

38.Nengqiang Wang, Jin Huang, Hongdou Luo, Pu Wang#, Jun Li (2013). Purification and characterization of a new carbonyl reductase from Leifsonia xyli HS0904 involved in stereoselective reduction of 3,5-bis(trifluoromethyl) acetophenone, Journal of Molecular Catalysis B: Enzymatic, 92:1-6. https://doi.org/10.1016/j.molcatb.2013.03.007.

39.Zhinan Xu#, Huili Zhang, Hao Chen, Feng Shi, Jin Huang, Shufang Wang, Cunjiang Song (2013). Microbial Production of Poly-γ-Glutamic Acid. In Bioprocessing Technologies in Biorefinery for Sustainable Production of Fuels, Chemicals, and Polymers (eds S.-T. Yang, H.A. El-Enshasy and N. Thongchul). https://doi.org/10.1002/9781118642047.ch23.

发明专利及获奖

1. 黄金,竺胜权, 唐万,杜美妮; 产酸克雷伯菌及其应用, 2020-12-25, 中国, ZL 201711112168.1;

2. 黄金, 王邵冰, 陈李, 修德恒; 一种长链二元酸精制装置, 2021-12-10, 中国, ZL202120321526.5;

3. 黄金, 王邵冰, 陈李, 修德恒 ; 一种长链二元酸精制装置的排料系统, 2022-1-7, 中国, ZL202120319563.2;

4. 黄金, 姜静, 王广雪; 阴沟肠杆菌Zjut HJ2001及其在发酵产低聚半乳糖中的应用, 2024-1-22, 中国, ZL2024012200065960;

5. 黄金, 姜静, 王广雪;一种乳糖不耐受症检测试剂盒, 2024-1-16, 中国, ZL202321242278.0;

6. 王普, 黄金, 丁徐中; 小孢根霉须状变种ZJPH1308及在制备西他列汀中间体中的应用, 2018-1-12, 中国, ZL201510289729.X ;

7. 王普, 金保军, 黄金, 孙婧, 何军邀; 近平滑假丝酵母ZJPH1305及在手性醇制备中的应用, 2016-3- 2, 中国, ZL201410040143.5 ;

8. 王普, 李军, 黄金; 红串红球菌XS1012及在制备手性醇中的应用, 2016-1-20, 中国, ZL201410024110.1 ;

9. 王普, 何军邀, 黄金, 梁法勇; 一种采用戊二醛交联固定化修饰脂肪酶Novozyme435的方法, 2014-6- 11, 中国, ZL201110460474.0;

10. 黄金(4/9); 西司他丁重要手性中间体的绿色合成关键技术研究及产业化开发, 浙江省科技厅, 科技进步, 省部二等奖, 2017;

行业标准

1.《二十二碳六烯酸油脂粉》  QB/T 5632-2021;

2.《花生四烯酸微胶囊》     QB/T 5631-2021;

3.《D-乳酸》                        QB/T 5937-2023;

其他

1.担任《Bioresource Technology》、《Applied Microbiology and Biotechnology》、《Journal of Chemical Technology and Biotechnology》、《Microbial Cell Factories》、《Process Biochemistry》、《Applied Biochemistry and Biotechnology》、《Engineering in Life Science》等SCI期刊国际审稿人，获得2018年度《Process Biochemistry》Outstanding Reviewer；

2. 国家自然科学基金、广东省自然科学基金、广东省科技厅项目、教育部学位中心学位论文等评审专家；

3. 主编教材《工业微生物实验技术教程》、参编《工业微生物分子生物学实验原理与技术》；