居述云，浙江大学工学博士，曾在浙江大学和美国加州大学圣巴巴拉分校开展博士后研究工作。2024年受聘为浙江工业大学药学院朝晖特聘研究员、运河青年学者，主要从事生命健康领域教学与科研工作。研究聚焦生物催化反应设计、酶的定向改造、化学-酶或多酶级联催化人工合成途径的组装与调控等方向。相关研究成果已发表于Nature Chemistry、Nature Communications、ACS Catalysis、Green Chemistry、化工进展等期刊，累计获授权国家发明专利11件，美国发明专利1件，参与编写生物催化专著2部。主持国家自然科学基金面上项目、浙江省自然科学基金探索项目、浙江省“尖兵”研发攻关计划项目子课题；作为项目骨干参与国家重点研发计划绿色生物制造专项。

The accelerating development of biocatalysis

学术论文：

Shuyun Ju^#, Dian Li^#, Binh Khanh Mai, Xin Liu, Alec Vallota-Eastman, Jianping Wu, David L. Valentine, Peng Liu* and Yang Yang*. Stereodivergent photobiocatalytic radical cyclisation through the repurposing and directed evolution of fatty acid photodecarboxylases. Nature Chemistry, 2024, 16, 1339–1347.

       Featured in Chemistry World: Light-driven enzyme engineered and repurposed to catalyse unnatural reaction.

    Highlighted by Benjamin List and Margareta M. Poje in Synfacts, 2024, 20 (7), 0746: Stereodivergent radical cyclizations  enabled by  directed evolution of fatty acid photodecarboxylases.

Shuyun Ju^#, Kaylee P. Kuzelka^#, Rui Guo, Benjamin Krohn-Hansen, Jianping Wu, Satish K. Nair^*, Yang Yang^*. A biocatalytic platform for asymmetric alkylation of α-keto acids by mining and engineering of methyltransferases. Nature Communications, 2023, 14(1), 5704.

Shuyun Ju, Xiaoshu Zhou, Huanhuan Jin, Yang Yang, Lirong Yang, Jianping Wu^*. Stereoselective oxidative C3-N bond dehydrogenation and aromatization of 1-carboxyl substituted tetrahydroisoquinolines employing pipecolate oxidase. Green Synthesis and Catalysis, 2024, https://doi.org/10.1016/j.gresc.2024.06.001.

Huanhuan Jin, Ziyuan Wang, Zhongji Pu, Haoran Yu, Yuhuan Chen, Xiaoshu Zhou, Lirong Yang, Shuyun Ju*, Jianping Wu*. Enantiodivergent kinetic resolution of 4-substituted 1,2,3,4-tetrahydroquinolines employing amine oxidase. International Journal of Biological Macromolecules, 2024, 269, 132102.

Huanhuan Jin, Shuyun Ju^*, Haoran Yu, Lirong Yang, Wenlong Zheng, Jianping Wu^*. An efficient biocatalytic oxidative dehydroaromatization approach for the construction of quinolines enabled by monoamine oxidase with molecular oxygen. Green Chemistry, 2023, 25(13), 5296–5303.

居述云, 吴坚平, 杨立荣^*. D-氨基酸氧化酶的分子改造及应用研究进展. 化工进展, 2021, 40(3), 1215–1225. [特约评述]

Tianxin Liang, Jun Sun, Shuyun Ju^*, Shenyi Su, Lirong Yang, Jianping Wu^*. Construction of T7-like expression system in Pseudomonas putida KT2440 to enhance the heterologous expression level. Frontiers in Chemistry, 2021, 9, 664967.

Guosi Li, Jiazhang Lian, Hailong Xue, Yiqi Jiang, Shuyun Ju, Mianbin Wu, Jianping Lin^*, Lirong Yang. Biocascade synthesis of L-tyrosine derivatives by coupling a thermophilic tyrosine phenol lyase and L-lactate oxidase. European Journal of Organic Chemistry, 2020, 8, 1050–1054.

Shuyun Ju, Mingxin Qian, Jing Li, Gang Xu, Lirong Yang, Jianping Wu^*. A biocatalytic redox cascade approach for one-pot deracemization of carboxyl-substituted tetrahydroisoquinolines by stereoinversion. Green Chemistry, 2019, 21(21), 5579–5585.

Shuyun Ju, Mingxin Qian, Gang Xu, Lirong Yang, Jianping Wu^*. Chemoenzymatic approach to (S)-1,2,3,4-tetrahydroisoquinoline carboxylic acids employing D-amino acid oxidase. Advanced Synthesis & Catalysis, 2019, 361(13), 3191–3199.

    Featured in Applied Biocatalysis: The Chemist's Enzyme Toolbox, 2020, Chapter 12, Chemo-Enzymatic Cascade: chemo-enzymatic synthesis of (S)-1,2,3,4-tetrahydroisoquinoline carboxylic acids.

An Huang, Xianheng Song, Linlin Song, Shuangshuang Pan, Jiaxin Tian, Pengfei Wang, Shuyun Ju, Yajun Wang*. Reshaping the substrate-binding pocket of ene-reductase for enhanced and inverted stereoselectivity: a concise access to the stereocomplementary chiral GABA derivatives. ACS Catalysis, 2025, 15, 1961–1972.

Jiaqi Zhu, Jianeng Xu, Xinru Bian, Peng Cheng, Zhe Dou, Wenting Dai, Shuyun Ju, Yajun Wang*. Enhancing the stability and activity of enzymes through layer-by-layer immobilization with nanocomposite hydrogel. Biochemical Engineering Journal, 2025, 215, 109604.

Lili Yao, Bin Xue, Yuanfan Ye, Zhixiu Wang, Yangyang Li, Beifengchu Zheng,  Shuyun Ju, Yajun Wang*. Structure-guided engineering of carbonyl reductase LbCR to simultaneously enhance catalytic activity and thermostability toward bulky ketones. Journal of Agricultural and Food Chemistry, 2025, 73, 10470–10481.

An Huang, Yanjun Wan, Xianheng Song, Wenmei Feng, Shuyun Ju, and Yajun Wang*. Combining Residue Substitution and Deletion Engineering Enhances the Substrate Acceptance of Ene-Reductase for Highly Enantioselective Synthesis of β‑Cyano Ester Scaffolds. ACS Sustainable Chemistry & Engineering, 2026, 14, 2965–2979.

J. Agric. Food Chem.2025, 73, 10470−10481J. Agric. Food Chem.2025, 73, 10470−10481Structure-Guided Engineering of Carbonyl ReductaseLbCR to
Simultaneously Enhance Catalytic Activity and Thermostability
toward Bulky KetonesStructure-Guided Engineering of Carbonyl ReductaseLbCR to
Simultaneously Enhance Catalytic Activity and Thermostability
toward Bulky Ketones

主持项目基金：

2026.01-2027.12               浙江省自然科学基金探索项目                                   

2026.01-2029.12               国家自然科学基金面上项目

2026.01-2029.12               大型仪器设备开放基金项目                                      

2025.01-2026.12               浙江省“尖兵领雁+X”科技计划项目子课题                       

2024.07-2029.07               浙江工业大学科研启动基金                                     

参讲：

本科生课程：酶工程

研究生课程：酶学与酶工程

参编：

生物催化剂工程原理及应用（主编：郁惠蕾，许建和），化学工业出版社，2025.

Applied Biocatalysis: The Chemist's Enzyme Toolbox (Editors: John Whittall & Peter W. Sutton), WILEY Press, 2021.