李树芳

李树芳，中国科学院天津工业生物技术研究所，博士，浙江工业大学生物工程学院教师。教育部全国高校黄大年式教师团队成员，浙江省生物有机合成技术重点实验室成员，浙江工业大学郑裕国院士创新团队成员。先后主持国家重点研发计划子课题1项，国家自然科学基金青年基金1项，参与国家自然基金面上项目2项。在Biotechnol Bioeng、Bioorg Chem、Crit Rev Biotechnol 等国内外期刊发表论文15篇，授权专利4项。

本科生： 酶工程 （浙江省线下一流课程）

      改变生活的生物技术

研究生： 酶学原理与酶工程

1. 国家重点研发子课题项目，  2023/12-2028/11，110 万，主持

2. 国家自然科学基金面上项目，2022/01-2025/12，60 万， 参与

3. 国家自然科学基金面上项目，2022/01-2025/12, 60 万，参与

4. 国家自然科学基金青年基金，2019/01-2021/12, 26 万，主持

5.国家自然科学基金青年项目，2014/01-2016/12，参加

代表性论文:

1. Li, S.F., Gao, YC, Xu, HB, et al. Substrate access tunnel engineering of a Fe-type nitrile hydratase from Pseudomonas fluorescens ZJUT001 for substrate preference adjustment and catalytic performance enhancement. Bioorg Chem. 2024;152:107744.

2. Li, S.F., Zhang W, Zhang W, et al. Recent Advances in the synthesis and analysis of Atorvastatin and its Intermediates. Curr Med Chem. 2024;31(37):6063-6083.

3. Li, S. F., Cheng, F., Wang, Y. J., Zheng, Y. G. Strategies for tailoring pH performances of glycoside hydrolases. Critical Reviews in Biotechnology, 2023, 43 (1): 121-141. doi: 10.1080/07388551.2021.2004084.

4. Li, S.F., Xu, S.Y., Wang, Y. J., Zheng, Y. G. Tailoring pullulanase PulAR from Anoxybacillus sp. AR-29 for enhanced catalytic performance by a structure-guided consensus approach. Bioresources and Bioprocessing, 2022, 9: 25.

5. Li, S. F., Xie, J. Y., Qiu, S., Xu, S. Y., Cheng, F., Wang, Y. J., Zheng, Y. G. Semi-rational engineering of an aldo-keto reductase KmAKR for overcoming trade-offs between catalytic activity and thermostability. Biotechnology and Bioengineering, 2021, 118 (11): 4441-4452.

6. Li, S. F., Xie, J. Y., Qiu, S., Zhou, S. Y., Wang, Y. J., Zheng, Y. G. Tailoring an aldo-keto reductase KmAKR for robust thermostability and catalytic efficiency by stepwise evolution and structure-guided consensus engineering. Bioorganic Chemistry, 2021, 109: 104712. 

7. Li, S. F., Xu, J.Y., Bao, Y. J., Zheng, H.C., Song H., Structure and sequence analysis-based engineering of pullulanase from Anoxybacillus sp. LM18-11 for improved thermostability, Journal of Biotechnology, 2015, 210: 8-1

8. Li, Y., Li, S. F., Zhang, L., Xue Y. P., Zheng, Y. G. Engineering of a hydroxysteroid dehydrogenase with simultaneous enhancement in activity and thermostability for efficient biosynthesis of ursodeoxycholic acid. Appl Environ Microbiol. 2024;90(9):e0123724. 

9. Zhang, W., Li, S. F., Zhu, J. Q., Cao, H.X., Liu, H. T., Shao, Z. Q., Xu, S. Y., Wang, Y. J., Zheng, Y. G. Constructing a continuous-flow bioreactor with co-immobilized KmAKR and BmGDH for synthesizing tert-butyl 6-cyano-(3R,5R)-dihydroxyhexanoate. Biochemical Engineering Journal, 2023, 197: 108964.

10. Qiu, S., Xu, S. Y., Li, S. F., Meng, K. M., Chen, F., Wang, Y. J., Zheng, Y. G. Fluorescence-based screening for engineered aldo-keto reductase KmAKR with improved catalytic performance and extended substrate scope. Biotechnol. J., 2021; 2100130.

11. Cheng F., Chen Y., Qiu S., Zhai Q. Y., Liu, H. T., Li, S. F., Weng, C. Y., Wang, Y. J., Zheng, Y. G. Controlling stereopreferences of carbonyl reductases for enantioselective synthesis of atorvastatin precursor. ACS Catal., 2021, 11 (5): 2572–2582. 

代表性专利：

1. 王亚军，邱帅，李树芳，程峰，翁春跃，郑裕国. 马克斯克鲁维酵母醛酮还原酶KmAKR 突变体及其应用. ZL 201910932502.0.

2. 王亚军，程峰，邱帅，李树芳，郑裕国. 醛酮还原酶KmAKR 突变体及其催化合成手性醇的应用. ZL 202110136118.7.

3. 王亚军，李树芳，邱帅，程峰，郑裕国. 醛酮还原酶KmAKR 突变体、工程菌及其应用. ZL 202110900178.1.