欧志敏

浙江工业大学药学院教授，博士生导师，硕士生导师。

2003年6月， 毕业于浙江大学生物化工专业，获工学博士学位。

承担课程：

本科生：“制药设备与车间设计”和“制药工艺课程设计”。

研究生：“生化反应工程与反应器”和“手性药物的生物合成（双语课程）”。

主持项目： 

1. 基于辅酶再生的纳米高分子-双酶结合体的理性设计及在油水两相中增效不对称还原反应的机制，国家自然科学基金面上项目。

2.化学药物制造关键技术研究-甾体药物生物合成的关键产业化技术开发，浙江省科技计划-尖兵研发攻关计划。

3.氧化还原酶的应用，浙江九洲药物科技有限公司，重大横向项目。

4.界面自组装羰基还原酶和辅酶的制备及其在油-水界面催化手性醇的合成，浙江省自然科学基金。

5.手性醇类药物中间体的生物法清洁合成，浙江九洲药物科技有限公司，重大横向项目。

6.酶活指纹谱法高通量筛选不对称还原芳香族β-羰基酯的高效抗体酶，浙江省自然科学基金。

7.抗抑郁药手性中间体β-羟基苯丙酸乙酯的生物合成新技术，浙江省科技厅科技计划项目。

8.β-葡萄糖苷酶高产菌株发酵法炮制牛蒡子的研究，浙江省中医药管理局项目。

9.《生物制药设备》校级优秀课程。

10.校级本科核心课程《制药设备与车间设计》、《制药工艺课程设计》。

11.校级研究生教改项目《手性药物生物合成》双语课程。

12.校级研究生实践教学改革项目。

13.校级重点专业建设课程“制药设备与车间设计”“生物制药工艺课程设计”，2023-2024年。

14.校级研究生在线课程建设项目《手性药物的生物合成》，2023-2025年。

15.全国药学教指委研究生精品课程《手性药物的生物合成》，2024年。

代表论文：

(1)Nana wang，Jiayu Teng，Yanmei Dai，Zijuan Tao，Changshun Huang，Liangli Luo，Zhimin Ou*. Semi-rational design of the nitroreductase EcNTR for the reduction of m-nitroacetophenone. Molecular Catalysis, 2025,6(580):115101

(2)Yanmei Dai†, Jinmei Wang†, Zijuan Tao, Liangli Luo, Changshun Huang, Bo Liu, Hanbing Shi, Lan Tang*,  Zhimin Ou* . Highly efficient synthesis of the chiral ACE inhibitor intermediate (R)-2-hydroxy-4-phenylbutyrate ethyl ester via engineered bienzyme coupled systems. Bioresources and bioprocessing. 2024, 11:99. 

(3) Zhenyu He†, Pengpeng Cheng†, Guoqing Ying, Zhimin Ou*. Research Progress in the Construction and Application of In Vitro Vascular Models. Appl. Sci. 2024, 14, 6780. https://doi.org/10.3390/app14156780

(4)Jian Xu, Hongwei Ye, Xindan Zhanf, Yangbin Lv, Shengqiang Tong, Biao Liu, Zhimin Ou*,  Chu Chu*. Comparative study on metabolite variations of two rose teas by plant metabolomics and revealing their skin-whitening candidates by spectrum–effect relationship analysis. Phytochemical Analysis. 2024.7.24. https://doi.org/10.1002/pca.3420

(5) Pengpeng Cheng† , Zhenyu He† , Bo Liu, Jinmei Wang，Chuyue Zhang, Lan Tang, Lihua Du, Yuan Lu* and Zhimin Ou*. 

Enhanced catalytic efficiency for chiral alcohol pharmaceutical  intermediate production using polyacrylic acid (PAA)-based nano bienzyme conjugates at the organic-buffer biphasic interface. ProcessBiochemistry,2024. doi: https://doi.org/10.1016/j.procbio.2024.06.001

(6)Mei Liu, Nana Wang, Lixia Deng, Bo Liu, Yanni Wang, Qingyu Zhang, Lan Tang, Qingqing Zhou, Jianhong Pan, Zhimin Ou*.  Dual enzyme self-assembly cluster for high efficient asymmetric reduction of ethyl 6-oxo-8-chlorooctanoate. Biochemical Engineering Journal, 2024, 207:109319

(7)Yuan Lu, Jinmei Wang, Pengpeng Cheng, Chuyue Zhang, Jinghua Li, Zhimin Ou*. Efficient biosynthesis of (R)− 1-[3,5-bis(trifluoromethyl)phenyl] ethanamine with self-assembled dual-enzyme clusters. Process Biochemistry, 2023,134:151–164

(8)Muhammad Naeem, Abdul Nasir, Mengjun Fang, Zhimin Ou*, Peijie Shen, Zhenjun Si, Zhinan Xu*. Characterization and rational engineering of Butyrivibrio crossotus argonaute for improved cleavage activity.Biochemical  Engineering Journal,2024,202:109113

(9)Yuan Lu, Jinmei Wang, Haobo Xu, Chuyue Zhang, Pengpeng Cheng, Lihua Du, Lan Tang, Jinghua Li, Zhimin Ou*. Efficient Synthesis of Key Chiral Intermediate in Painkillers (R)-1-[3,5-Bis(trifluoromethyl)phenyl]ethanamine by Bienzyme Cascade System with R-ω-T ransaminase and Alcohol Dehydrogenase Functions. Molecules, 2022, 27(21),

7331-7348.

(10)Jinmei Wang, Yuan Lu, Pengpeng Cheng, Chuyue Zhang, Lan Tang, Lihua Du, Jinghua Li, Zhimin Ou*.  Construction of Bi-Enzyme Self-Assembly Clusters Based on SpyCatcher/SpyTag for the Efficient Biosynthesis of (R)-Ethyl 2-hydroxy-4-phenylbutyrate. Biomolecules, 2023,13,91.DOI：10.3390/biom13010091.

(11)Xin-Yuan Liu, Yi-Li Chen, Guo-Jian Liu, Xiang-Nan Deng, Yue Cui, Jie Tan, Xing-Chen Dong, Hua-Ying Li, Gan-Jun Chen, Zhimin Ou*, Chun-He Wang. Development of a variant of dinutuximab with enhanced antitumor efficacy and reduced induction of neuropathic pain. FEBS Open Bio, 2022,12(9):1664-1656.

(12) Hongqian Dai; Yuan Lu; Hanbing Shi; Lan Tang; Xingyuan Sun; Zhimin Ou*. Efficient enantiomer selective acetylation of 1-methyl-3-phenylpropylamine by Fe3O4-APTES-CS2-lipase magnetic  nano particles in an alternating magnetic field, Biocatalysis and Biotransformation,  2021, 10.1080/102 42422.202(10.1080/10242422.202)  

(13)Yuan Lu; Hongqian Dai; Pengpeng Cheng; Hanbing Shi; Lan Tang; Xingyuan Sun;  Zhimin Ou*. Regenerated coenzyme-based preparation of bienzyme-polymer nanoconjugates and their applications f or the synthesis of ethyl (R)-2-hydroxy-4-phenylbutyrate, Korean J. Chem. Eng., 2021.春季, 38(5): 1066-1077 

(14)Yuan Lu, Hongqian Dai, Hanbing Shi, Lan Tang, Xingyuan Sun, Zhimin Ou*. Synthesis of ethyl (R)-4-chloro-3-hydroxybutyrate by immobilized cells using amino acid-modified magnetic nanoparticles. Process Biochemistry, 2020,99:9–20. 

(15)Zhimin Ou*; Jiaying Pan; Lan Tang; Hanbing Shi. Continuous enantiomer-selective acylation reaction of 1-phenylethanamine in a magnetic fluidized bed reactor system(MFBRS),Journal of Chemical Technology and Biotechnology, 2019, 94: 1951-1957 

(16)Zhimin Ou*, Jiahui Xu, Lihua Du, Lan Tang, Yangping Niu, Jian Cui. Preparation of Interfa ce-Assembled  Carbonyl Reductase and Its Application in the Synthesis of S-Licarbazepine in Toluen e/Tris-HCl Buffer Biphasic System, Journal of Microbiology and Biotechnology, 2018, 28(4): 613-621  

(17)Jiaying Pan, Zhimin Ou*, Lan Tang, Hanbing Shi. Enhancement of catalytic activity of lipa se-immobilized Fe 3 O 4 -chitosan microsphere for enantioselective acetylation of racemic 1-pheny lethylamine, Korean Journal of Chemical Engineering, 2019, 36(5): 729-739 

(18) Zhimin Ou*, Lan Ma, Yangpin Niu, Jian Cui. Preparation of (R)-(-)-mandelic acid by two-step biotransformation of ethyl benzoylformate. Biocatalysis and Biotransformation, 2018,36(6):409-416

(19)Zhimin Ou*, Han-bing Shi, Xing-yuan Sun, Wen-he Shen. Synthesis of SLicarbazepine by asymmetric reduction of oxcarbazepine with Saccharomyces cerevisiae CGMCC No. 2266.  

Journal of Molecular Catalysis B: Enzymatic, 2011,72(3-4):294-297.

(20) Zhimin Ou*, Xiaoyan Chen, Guoqing Ying, Hanbing Shi, Xingyuan Sun. Continuous preparation of (S)-3-hydroxy-3-phenylpropionate by asymmetric reduction of 3-oxo-3-phenyl-propionic acid ethyl ester with Saccharomyces cerevisiae CGMCC No. 2266 in a membrane reactor.  Biotechnology and Bioprocess Engineering, 2011,16(2):320-326.

(21)Z. Ou*, G. Yang. Asymmetric reduction of  Carbonyl phenylpropionate by undifferentiated cells of white turnip in phosphate buffer/organic solvent. New Biotechnology,2009,25:s140.

(22)Zhimin Ou*, Jianping Wu, Lirong Yang, Peilin Cen. Asymmetric reduction of chloroacetophenones to produce chiral alcohols with 

microorganisms. Korean Journal of Chemical Engineering,2008,25(1):124-128.

(23) OU Zhimin*, GUO Fangyuan, YANG Gensheng, YAO Shanjing. Enhancement of arctigenin content by processing of  Fructus Arctii via solid-state fermentation coupled with poach. Chinese Journal of Catalysis,2009,30(1):48-52.

(24)OU Zhimin*, YANG Gensheng, GUO Fangyuan. Preparation of (S)-(-)-β-hydroxy-benzenepro-anoic acid ethyl ester by asymmetric reduction of  β-Carbonyl phenylpropionate with immobilized CGMCC No.2266. 

Huagong Xuebao / CIES Journal, 2009,60(2):421-427.